The wineasio DLL must be registered in the wineprefix. For both 32 and 64 bit wine do: # regsvr32 wineasio.dll On a 64 bit system with wine supporting both 32 and 64 applications, regsrv32 will register the 32 bit driver in a 64 bit prefix, use the following command to register the 64 bit driver in a 64 bit wineprefix: # wine64 regsvr32 wineaiso.dll Note: regsvr32 registers the ASIO COM object in the default prefix "~/.wine". To use another prefix specify it explicitly, like: # env WINEPREFIX=~/asioapp regsvr32 wineasio.dll